FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n02_ATAC1_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n02_ATAC1_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29944270
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330140.11025147716073892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC94400.025.2674925
TTGGGAG572950.024.6400666
CGAGTAG135350.024.561143
CCGAGTA136000.024.5554942
AGGCCGA173600.024.39571211
GTAGCTG473300.024.3745386
ATCGCTT115250.024.31433527
AGTAGCT478800.024.2724975
GGGATTA649350.024.15791912
CCCGAGT144150.024.1394631
GGATTAC609050.024.12436313
AGGCTGA577200.024.10677111
GATTACA618100.023.98027214
CGCTTGA113050.023.9521229
TCGCTTG114250.023.9306128
TTTGGGA459250.023.830695
TGGGATT674250.023.79588911
ACTTTGG420500.023.6286723
TACAGGC552400.023.58979817
ATTACAG624800.023.48508815
Produced by FastQC (version 0.11.5)