FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n02_ATAC12_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n02_ATAC12_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25769913
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG284340.11033797436568761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA116750.024.8895452
CGAGTAG116200.024.7951153
GAATCGC77200.024.2524225
GGGATTA526350.024.14237812
AGGCCGA132750.024.0546411
GGATTAC500250.024.03777513
AATCGCT79900.024.01477226
AGTAGCT407750.023.9920315
GTAGCTG405100.023.873846
GATTACA511550.023.74229814
TTGGGAG473950.023.7287256
ATCGCTT93900.023.686627
AGGCTGA479600.023.50071711
GAGTAGC261250.023.4002114
ATTACAG515400.023.31830415
TGGGATT559250.023.29965811
TCGCTTG93600.023.23293928
TTTGGGA373850.023.2112375
CGCTTGA92850.023.18716829
ACTTTGG336600.023.1650373
Produced by FastQC (version 0.11.5)