FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n02_ATAC11_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n02_ATAC11_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28640646
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG121850.024.3963833
CCGAGTA122200.024.2099482
GAATCGC82400.024.03871725
AGGCCGA141650.023.91138511
GGATTAC529750.023.8818613
GGGATTA571250.023.65897412
AGTAGCT428550.023.6488195
TTGGGAG501600.023.5981256
GTAGCTG425400.023.5508066
GATTACA547400.023.49303614
AATCGCT86900.023.382426
AGGCTGA518150.023.12265211
ATCGCTT103600.023.11419127
ATTACAG553950.023.00819615
AGAATCG90000.022.9213924
TTTGGGA395400.022.9068455
TGGGATT604550.022.87960611
CCCGAGT134100.022.7471371
GAGTAGC284950.022.7217314
TACAGGC498150.022.71409417