FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46367346
Sequences flagged as poor quality0
Sequence length37
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20994294.527817917376596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG10750.028.284681
CGATCGT11000.027.7657342
GATCGTC13650.025.2071828
CGAGTAG47650.024.4644153
GTAGCTG156850.024.3410666
AGTAGCT160050.024.2128775
CGCGGTT18650.024.1034326
GATTACA200450.023.82349814
AGGCCGA50100.023.60468711
TACAGGC182700.023.52432617
TTACAGG191350.023.4818416
ATTACAG204000.023.43133715
CGATCAT14850.023.2960381
CCGAGTA50050.023.2942832
AGGCTGA183550.023.28058411
TTTGGGA132600.023.0645835
ATGATCG15100.022.994866
CTGGGAT214250.022.94666510
GAGTAGC109450.022.8585114
ATCGTCT15000.022.8351869
Produced by FastQC (version 0.11.5)