FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n01_ATAC5_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n01_ATAC5_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27239267
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC100800.025.9404825
CGAGTAG145050.025.7658443
CCGAGTA146350.025.3996052
ATCGCTT122100.025.05853727
AGGCCGA170900.024.92269111
TTGGGAG569550.024.9058176
CCCGAGT151550.024.7578431
AGTAGCT489600.024.6890475
GTAGCTG484700.024.6828826
AATCGCT108150.024.59315326
TTTGGGA453000.024.5553845
CGCTTGA120600.024.54780829
AGGCTGA585350.024.4747111
GGATTAC606200.024.47178313
GGGATTA638900.024.43236512
TCGCTTG121150.024.41050728
GATTACA615900.024.3255814
ACTTTGG413200.024.238423
TGGGATT668650.024.04505511
ATTACAG621650.023.96567515
Produced by FastQC (version 0.11.5)