FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n01_ATAC3_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n01_ATAC3_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30707271
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG161900.025.1998943
CCGAGTA162750.025.1732962
AGGCCGA189550.024.9400211
GAATCGC107450.024.71010225
GGATTAC666600.024.57504513
GGGATTA708000.024.50411212
GTAGCTG548300.024.4911026
AGTAGCT551600.024.4373175
GATTACA681950.024.40843214
TTGGGAG633000.024.3484886
ATCGCTT128450.024.31465127
CCCGAGT172700.024.228831
AGGCTGA654300.024.21947311
AATCGCT111650.024.1971226
TGGGATT737150.024.16774211
CGTGAGC155300.024.09280223
ATTACAG689150.024.00474415
CGCTTGA125700.023.93431329
TTTGGGA499750.023.8182665
TACAGGC628100.023.77368517