FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n01_ATAC2_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n01_ATAC2_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26895632
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC98550.026.04481125
CGAGTAG152350.025.8847453
CCGAGTA152400.025.7036692
AGGCCGA169500.025.38470611
AATCGCT103500.025.23352426
CCCGAGT158350.025.1832941
GGGATTA672700.025.03891212
AGTAGCT522450.024.9944085
GTAGCTG519150.024.9830886
GGATTAC640050.024.93346613
TTGGGAG584300.024.871656
GATTACA652100.024.85107214
ATCGCTT122050.024.71292927
TGGGATT700800.024.66057211
AGGCTGA595650.024.5850511
ATTACAG652800.024.5819615
TTTGGGA463450.024.4636525
TCGCTTG119650.024.41834428
CGCTTGA119200.024.3544929
CGTGAGC145050.024.29869723