FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n01_ATAC1_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n01_ATAC1_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29944270
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC99750.025.1255325
AGGCCGA169100.024.72068811
CGAGTAG143350.024.687873
TTGGGAG568300.024.5274626
CCGAGTA144150.024.411592
ATCGCTT122750.024.36999327
GTAGCTG498250.024.3544036
AGTAGCT501900.024.2823415
CGTGAGC143000.024.27867723
CGCTTGA119900.024.27685229
GGGATTA660300.024.2226112
GGATTAC622900.024.1641613
CCCGAGT148200.024.1254271
TTTGGGA445750.024.0686745
AATCGCT107100.024.05247126
GATTACA636150.024.00456614
TCGCTTG121650.023.991428
AGGCTGA590600.023.97912811
TGGGATT687450.023.93327311
ATTACAG642750.023.62046615