FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n01_ATAC11_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n01_ATAC11_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28640646
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC89500.024.92035525
CGAGTAG127050.024.59713
CCGAGTA129600.024.1733112
AGTAGCT436000.023.7637485
GGGATTA573550.023.73543212
AATCGCT97100.023.7043626
GGATTAC536100.023.69338813
GTAGCTG433650.023.6459226
GATTACA552250.023.52282514
AGAATCG98450.023.33171524
AGGCCGA141250.023.20827711
TTGGGAG504900.023.1336216
CGCTTGA106950.023.10140629
ATTACAG557050.023.0945515
ATCGCTT111150.023.07864427
AGGCTGA540900.023.07606311
TGGGATT609950.023.03787811
CCCGAGT141900.023.0001681
CGTGAGC130050.022.93009623
GAGTAGC287200.022.9116694