FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13935909
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9921517.119384892653935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT19200.023.5702841
ACTCTAG29350.023.3682825
CTAGCAG22300.019.9214178
CTGACTC23550.019.3197482
GTGCGCA22800.016.91407468
AGGGGGG11900.015.8790915
CTCTAGC30800.015.7919846
TGCGCAT25100.015.22559969
AGGTGCG26250.015.22464766
TAGCAGA29800.015.1422419
CTATCGA31000.014.10165117
TCTATCG31150.014.03339416
GAGGGGG12850.013.615934
CTAGTTG15050.013.4807158
TACTCTA15400.013.1791584
TCTAGCA54500.013.1584197
CGGGAAT43200.012.9930941
TCTAGTT20200.012.4688817
GATCTAT36650.012.21368514
GGGGAGG17700.012.0901851
Produced by FastQC (version 0.11.5)