FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13727660
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT227560.1657675088106786No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT220370.16052990822907912No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA206430.15037522782469845No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA190080.13846496780951745No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT145020.10564072828144054No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG143800.10475201163198972No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGC138950.10121899872228769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG110950.033.5354545
GCTGACT55400.030.3990571
CTAGTTG74450.027.2644468
CTCTAGT81550.026.1821756
TCTAGTT81700.026.0443387
CTAGCAG68750.024.7907498
GACTCTA69000.024.7552554
TACTCTA83900.024.5308174
CTGACTC71050.023.5487772
ACCTCTA86250.023.33147217
CTAATGC86300.023.1156121
GTTACCT87600.022.77215414
GTACTCT91650.022.380173
GGACACC86700.022.24207929
GACACCT89250.021.48887330
TGTTACC94950.020.97252313
TACCTCT95800.020.93256816
ATGCTGG94500.020.81360824
TTACCTC97000.020.7096115
TGGACAC96850.020.23608628
Produced by FastQC (version 0.11.5)