FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12842602
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT244900.19069344358721077No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA232520.1810536525230635No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT197900.1540964985133075No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG177100.13790040367209075No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGC160380.12488123512665113No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG145780.11351282240156628No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT143910.11205673118266843No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC143510.11174526782033734No Hit
ATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGA129320.10069610504164186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT50900.031.2261521
ACTCTAG80600.028.0105845
GACTCTA67050.024.7443144
CTAGCAG66000.024.5509688
CTGACTC68750.023.4196822
CTCTAGC87050.019.3810586
TAGCAGA89900.018.1798559
CTAGTTG38350.017.703868
TGACTCT94300.017.519613
TCTAGTT46600.016.822497
CTATCGA95550.016.51850117
TCTATCG98800.016.32934616
TCTAGCA171350.016.0738097
TACTCTA41150.015.8211824
GGACACC39950.015.50570229
GACACCT41050.015.34596930
CTCTAGT48450.015.3158576
CTAATGC43050.015.03926921
CGGGGGT8950.014.8641791
GATCTAT107100.014.80256314
Produced by FastQC (version 0.11.5)