FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11955606
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA218290.18258380210923647No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT214290.17923809131883403No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT178430.14924379408287627No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG169800.14202542305258303No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGC142950.11956733937200674No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC134510.11250788960425762No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG134290.11232387551078549No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT130330.10901162182828708No Hit
ATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGA122730.10265477132652247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG74700.029.8007935
GCTGACT50200.029.7773671
CTAGCAG58400.025.7087678
GACTCTA60350.025.577074
CTGACTC61700.024.5065752
CTCTAGC80050.019.5013566
CTAGTTG36350.019.4483938
TAGCAGA76750.019.4253949
TCTAGTT44700.017.7726967
TGACTCT88450.017.5698953
TACTCTA43800.016.7017384
CTCTAGT45950.016.6058946
GGACACC39500.016.47989729
CTATCGA87950.016.43422317
TCTAGCA155350.016.4229557
CTAATGC41700.016.0302921
TCTATCG89750.015.98750816
GACACCT43200.015.71659430
GTTACCT43400.015.56307214
ACCTCTA44500.015.41455717
Produced by FastQC (version 0.11.5)