FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13345322
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG327240.2452095198602177No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT199180.14925080114215306No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC170730.12793246952003107No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG168420.12620152589798883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG95750.030.0848465
GCTGACT55700.028.3451041
CTAGCAG61350.026.1839038
CTAGTTG55800.023.7079438
GACTCTA71800.023.301754
CTGACTC68300.023.265922
TCTAGTT62200.021.7185887
TACTCTA60300.021.4187534
CTCTAGT67700.020.3184536
CTAATGC62300.020.22308721
GTGCGCA127250.020.13770768
CTCTAGC84950.019.7771916
TGCGCAT131300.019.62327469
GTTACCT65400.019.47840714
ACCTCTA65350.019.43982917
GTACTCT68750.019.14253
TAGCAGA84300.019.0969899
GACACCT64550.018.7595630
GGACACC64150.018.65830829
GGTGCGC139400.018.43260867
Produced by FastQC (version 0.11.5)