FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11840764
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG292300.24685907091805903No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT191730.16192367316838677No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC183280.15478730933240456No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG170500.14399408686804333No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG134560.11364131571239829No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC127280.10749306379216747No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA124740.10534793194087813No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC123210.10405578558951095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT48450.028.6879731
ACTCTAG74800.028.2637795
CTAGCAG60050.023.9526778
CTGACTC59250.023.925532
GACTCTA62500.023.297444
GTGCGCA112250.019.11799868
CTAGTTG36550.018.6712078
TGCGCAT116900.018.38763269
TACTCTA38250.018.3017864
TCTAGTT41350.018.2810697
CTCTAGC81450.018.2208166
TAGCAGA80850.018.1801019
GGTGCGC119950.017.86103667
CTAATGC38950.017.43132821
AGGTGCG124500.017.09573766
GGACACC37650.017.0112829
TGACTCT87850.016.9730193
ACCTCTA41350.016.67350617
GACACCT39950.016.46986830
CTATCGA84900.016.44755717
Produced by FastQC (version 0.11.5)