FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10680845
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG250680.23470053165269228No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT178800.1674024854775067No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC168890.15812419335736078No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG145350.13608473861384562No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG117660.11015982349711095No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA115440.1080813362613164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG71850.031.6163775
GCTGACT46200.030.3137951
CTAGCAG52650.026.5886828
CTGACTC54550.025.3452452
GACTCTA58100.024.9411434
TCTAGTT41950.021.7742777
CTAGTTG38900.021.6821638
TAGCAGA68450.020.6047239
CTCTAGC72850.020.2757576
TACTCTA42600.019.7194044
CTCTAGT47250.019.112266
GTGCGCA94600.019.05773468
GTTACCT44550.018.93252814
TGCGCAT97300.018.5649669
GGACACC42800.018.48051629
GACACCT42900.018.35585630
CTAATGC44900.018.3172721
ACCTCTA45350.018.28977217
ATGCTGG45200.018.11826724
GGTGCGC102400.017.94751567
Produced by FastQC (version 0.11.5)