FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11743086
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT138540.11797580295332931No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA133660.11382016618118951No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT122850.10461474947897002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG64700.025.8592155
GCTGACT42850.022.7961561
CTAGCAG48900.019.5382798
GACTCTA54200.018.9216944
TACTCTA39600.018.2080634
CTGACTC55000.017.8198362
CTAGTTG41250.017.6470418
TCTAGTT46750.016.394317
GGACACC41100.015.66808429
CTCTAGT49800.015.5329746
ACCTCTA44250.015.34344317
TAGCAGA67200.014.8945519
CTCTAGC68700.014.8770846
CTAATGC45700.014.78022421
GTTACCT46250.014.7555514
GACACCT43900.014.74853830
TCTAGCA114050.014.4222567
GTACTCT49150.014.314413
ATGCTGG47050.014.05854124
CTATCGA68350.014.02963717
Produced by FastQC (version 0.11.5)