FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10943742
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT138250.12632790502553878No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT129960.11875279954516471No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA115710.10573165924415981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG68400.027.6830045
GCTGACT42350.025.8730931
CTAGCAG46550.022.9304498
GACTCTA51900.021.7824764
CTGACTC51400.021.0412432
CTAGTTG42150.019.761138
TCTAGTT46100.018.902837
TACTCTA43600.018.3831984
CTCTAGT52550.017.2503996
TAGCAGA64850.017.2153979
CTCTAGC68400.016.732616
CTAATGC46800.016.4517921
ACCTCTA48750.016.22401217
GTACTCT50950.016.0748793
GGACACC48050.015.95082429
GTTACCT49250.015.91740214
CTATCGA68200.015.65092117
TCTAGCA114400.015.5711317
TCTATCG68900.015.44119116
ATGCTGG50000.015.3288824
Produced by FastQC (version 0.11.5)