FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11444141
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT127520.11142819718841282No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA121600.10625524449585162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG64700.024.8311355
GCTGACT41700.023.4232641
CTAGCAG48200.020.5478738
GACTCTA53950.019.8526364
CTGACTC55200.018.4522672
CTAGTTG38250.016.1030718
CTCTAGC70350.015.22458656
TCTAGTT44250.014.8686677
TAGCAGA67000.014.7822019
CTAATGC40100.014.40052821
TACTCTA41450.014.35530954
CTCTAGT48200.013.8699396
TCTAGCA117700.013.76676757
CTATCGA72400.013.68001917
GGACACC38950.013.65779729
TGACTCT82150.013.3786463
ACCTCTA44200.013.22309217
TCTATCG74900.012.94294216
ATGCTGG44350.012.78386424
GTTACCT44400.012.53272214
Produced by FastQC (version 0.11.5)