FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11465051
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT193290.16859061507881648No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT189270.1650843070824543No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA177920.15518465639620793No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG139180.12139501167504618No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC130600.1139113990857956No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGC117540.10252025917721604No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA116930.10198820746632528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG81450.031.6734225
GCTGACT52100.031.4510171
CTAGCAG60100.027.8347158
CTGACTC60950.027.0503772
GACTCTA63700.026.7613564
TACTCTA40400.023.6537424
CTCTAGC78500.021.8050846
CTAGTTG45200.021.4474018
TAGCAGA80300.021.0942969
TCTAGTT49350.020.7075657
GTACTCT47850.020.1905253
TGACTCT91550.019.2704853
GGACACC46700.019.26003829
GTTACCT48100.019.20861214
CTAATGC48500.018.83346421
CTCTAGT53850.018.6558386
ACCTCTA49900.018.51571717
TCTATCG89750.018.17140816
GACACCT49350.018.15489430
CTATCGA91500.017.97686217
Produced by FastQC (version 0.11.5)