FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14783863
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT177710.12020538880805375No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT172910.11695860547409023No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA152870.10340328505479252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT53550.025.8239251
ACTCTAG72050.023.6584155
GACTCTA66250.021.7142644
CTAGCAG65850.021.4186178
CTGACTC75200.018.804362
CTCTAGC90350.016.4645526
TAGCAGA88900.016.1408799
TCTAGCA159750.015.5326167
CTATCGA92200.015.10771217
TCTATCG95550.014.39489216
TGACTCT106700.014.3681323
GATCTAT109550.012.65108914
CGGGAAT136300.012.4318041
ATCTATC114250.012.161201515
CTAGCAT110850.012.15534226
CTTACAG125700.012.1114932
GCAGATC116500.012.01649211
TGCCGAG127400.011.86955265
CATGCCG124000.011.74361863
AGATCTA124150.011.64259613
Produced by FastQC (version 0.11.5)