FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16065353
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT186400.11602608420742452No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA180680.11246562711693918No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT171070.10648381022191047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT57350.024.0516241
ACTCTAG80450.022.5361585
GACTCTA71200.021.0397224
CTAGCAG67400.020.7700278
CTGACTC74300.019.031492
CTCTAGC97000.015.2993516
CTATCGA93450.015.01779417
TAGCAGA99700.014.4623579
TCTAGCA160500.014.2606627
TCTATCG98050.014.09907216
TGACTCT107500.013.7072763
CGGGAAT143500.013.0034011
GATCTAT111350.012.41495814
ATCTATC113100.012.28474715
CTAGCAT109250.012.109200526
CTTACAG128250.011.92540732
GGACAGC114150.011.49742140
CATGCCG128400.011.47800263
CGGGGGT12400.011.2932771
GCAGATC124500.011.24414211
Produced by FastQC (version 0.11.5)