FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17415381
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA193610.11117184286694617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG79500.019.9442925
GCTGACT58600.019.7152541
CTAGCAG70550.016.8663888
GACTCTA75150.016.860374
CTGACTC81100.014.6307452
TCTAGCA163000.012.9254777
CTCTAGC103750.012.8198176
TAGCAGA99750.012.2448299
CTATCGA95550.011.86733617
CGGGAAT141950.011.4190951
TCTATCG100000.011.19918516
TGACTCT119100.011.1381673
CTAGCAT107750.010.42627526
CTTACAG124250.010.36565132
CGGGGAT30000.010.2694571
CATGCCG124050.010.04602963
CGCCCAT20150.09.9034361
TGCCGAG127500.09.85664765
GCCGGAT43350.09.77197551
GCGGAAT29150.09.7282011
Produced by FastQC (version 0.11.5)