FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16363807
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT190840.11662322832333577No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA181650.11100717577517262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT54800.023.1910841
ACTCTAG82450.021.9897985
GACTCTA70200.019.0461444
CTAGCAG69850.018.4882438
CTGACTC72250.017.6822362
CTCTAGC94000.014.3728956
TAGCAGA95700.013.6040039
CTATCGA93850.013.23826517
TCTAGCA163250.013.1628397
CTAGTTG44400.013.0846118
TCTATCG96850.012.90043416
CGGGGGT11450.012.2306481
CGGGAAT149100.012.210141
TGACTCT111650.012.0379343
CGGGGAT27550.011.9454151
TACTCTA47000.011.8407754
CTTACAG131150.011.76816232
TCTAGTT57200.011.6861577
GCGGGAT31900.011.6334941
GGACACC43200.011.42272529
Produced by FastQC (version 0.11.5)