FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11610157
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT133720.1151750144291761No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA130700.1125738437473326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG61850.023.5988925
GCTGACT42800.023.1497571
CTAGCAG49950.019.7583628
GACTCTA53850.018.9148354
TATGCCG17450.018.85285848
CTGACTC52800.018.7606472
CGTATGC19200.017.68126746
GTATGCC20350.016.51010147
GCACACG21850.015.69676111
CTCTAGC68550.015.26705556
TACTCTA37000.014.379284
CTATCGA67250.014.10327317
TAGCAGA70600.014.0783239
TCTATCG68400.014.07076416
CTAGTTG34800.013.9788948
TCTAGCA112600.013.831177
GTCACGT25550.013.56091629
ACGTCTG26000.013.46058615
TGACTCT77700.013.2440173
AGTCACG26200.013.09090228
Produced by FastQC (version 0.11.5)