FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11736364
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG228160.19440433169932358No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT130300.1110224597669261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG64100.024.2987235
GCTGACT40950.024.2801551
CGTCCGC14000.023.24854333
GTCCGCA15650.020.3506834
CTAGCAG49850.020.148938
GACTCTA55850.019.8663274
GTATGCC17300.019.01590347
CTGACTC57200.018.2348292
GTGCGCA97200.018.00804968
TATGCCG18600.017.68706148
GGTGCGC101200.017.33056467
ACGTCCG19500.017.22946732
TGCGCAT103050.017.15561569
TCCGCAC18800.017.12702435
GCACACG20050.017.10586711
CGCACAT17550.016.75144237
AGGTGCG105800.016.54397466
CCGCACA18850.016.33884436
CGTATGC20700.016.06158846
CTAGTTG34100.014.5736798
Produced by FastQC (version 0.11.5)