FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12100506
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG207300.1713151499614975No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC142610.117854575668158No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT130010.10744178797151127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG71700.027.0436365
GCTGACT44750.024.3315241
CTAGCAG48450.021.7424148
GACTCTA54700.020.2196794
CTGACTC55600.019.8925482
CTAGTTG44450.019.7622768
TACTCTA46550.018.947654
TCTAGTT53850.017.8721437
GTGCGCA82750.017.4715768
TATGCCG19000.017.13041946
GGACACC48500.016.7414129
CGTATGC20750.016.69759644
GACACCT51150.016.55829230
TGCGCAT87800.016.50673169
GTATGCC19750.016.47989745
GGTGCGC88300.016.45222767
GTACTCT55000.016.3548563
CTCTAGT59900.016.127126
CTAATGC52900.016.07641621
TGTACTC56400.015.8868922
Produced by FastQC (version 0.11.5)