FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13665731
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG213610.15631070156437296No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA144760.1059292034944929No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC144650.10584871017876761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC21000.038.831635
GTCCGCA25300.032.9236736
CCCGTCC26200.031.39115333
CACCCGT26950.029.99778231
CCGTCCG27800.029.83679834
GTATGCC26400.028.10418947
TATGCCG27000.027.09098648
TCACCCG30400.026.82357830
CGTATGC28650.026.50783346
GCACACG36200.024.74964111
ACGTCTG37750.023.45547715
ACTCTAG80100.023.1156865
GTCACCC35600.023.1023129
TCCGCAT34250.023.09402837
ACCCGTC35950.022.58542332
CACACGT43300.020.85296212
ACACGTC43100.020.78747713
GCTGACT48800.020.660261
CTCGTAT35100.020.14118244
ATGCCGT36750.019.90372849
Produced by FastQC (version 0.11.5)