FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10677653
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG159790.14964899121557892No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT132670.12425015122705335TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG38550.041.66971211
GTATGCC26300.041.25115647
TATGCCG26800.039.17642248
ACGTCTG41800.038.34593615
ACACGTC42500.037.87940213
CGTATGC28250.037.4124946
CACACGT44550.036.13618512
AGCACAC46550.034.80899410
CACGTCT46050.034.73111714
GTCACAT51200.030.8966129
CTCGTAT34550.030.79297344
ATGCCGT34100.030.48192649
CAGTCAC52500.030.26473427
CGTCTGA53600.030.10026716
ATGTCAG53600.029.5131834
GAGCACA55950.029.211179
TCGTATG34850.029.02172345
CCAGTCA54800.028.99409326
TCCAGTC57300.027.79002825
CACATGT58650.027.50902731
Produced by FastQC (version 0.11.5)