FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_138.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_138.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9927814
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG159120.1602769753744379No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT101790.10253012395276544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT38950.024.5369191
ACTCTAG59700.024.1549855
CTAGCAG41750.022.3816838
GACTCTA47800.020.0633934
CTGACTC47850.019.9690782
GTGCGCA70150.016.9169368
CTATCGA56150.016.89099717
TCTATCG58000.016.17113116
TGCGCAT75500.015.99645869
TACTCTA32500.015.8312674
TAGCAGA60600.015.8239859
CTCTAGC65650.015.6746796
GGTGCGC76400.015.670320567
CTAGTTG33000.015.2716688
AGGTGCG80250.015.04962866
TAGCATG63550.014.42865627
GATCTAT66150.014.33755814
TCTAGCA101300.014.3375327
CTAGCAT65600.014.08453226
CGGGGGG8250.014.0031191
Produced by FastQC (version 0.11.5)