FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11415449
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG188740.1653373424032642No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT137530.12047708329300055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT42800.025.600851
ACTCTAG66700.024.8203145
GACTCTA54200.021.4393084
CTAGCAG51900.020.971478
CTGACTC57100.019.4310862
GTGCGCA86500.015.90487568
CTCTAGC74150.015.7656216
CTATCGA70250.015.294373517
TCTATCG71450.015.28234916
TAGCAGA74050.015.1239269
TGCGCAT91450.015.04410769
CTAGTTG35400.015.0271348
GGTGCGC91550.014.91230767
TACTCTA35500.014.8874594
AGGTGCG96600.014.27774366
CACCTTA37550.014.1667257
TGACTCT83400.014.1008473
TCTAGCA121500.013.7685267
TACCCTA37700.013.27540140
TCTAGTT45500.013.2297887
Produced by FastQC (version 0.11.5)