FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12949702
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG217260.16777220047225797No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT129670.1001335783634249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT46600.023.6660041
ACTCTAG66750.019.87385
CTAGCAG53050.019.7249878
GACTCTA61500.018.3830264
CTGACTC62000.017.2185292
GTGCGCA97150.017.18933768
TGCGCAT103450.016.17661569
GGTGCGC103750.016.16277167
AGGTGCG108800.015.347980566
TAGCAGA77550.014.2606039
TGACTCT86700.013.9280273
CTATCGA73550.013.89401417
CTCTAGC82900.013.679976
TCTAGCA133500.012.8715597
AAGGTGC135500.012.73701665
AGCGCGC36450.012.67728961
CCGGGGT11350.012.6470231
GCGCATG139850.012.64224470
TCTATCG80400.012.62325116
ATGCGCT141000.012.01436356
Produced by FastQC (version 0.11.5)