FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_135-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_135-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14772888
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG227080.1537140198991558No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT155890.1055243903561714No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC151800.10275580509376366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG82500.021.976915
GCTGACT55950.021.3364181
CTAGCAG59700.019.5209948
GACTCTA65500.019.130714
CTGACTC69650.016.734662
GTGCGCA102100.015.63588768
TGCGCAT110000.014.60852569
GGTGCGC111050.014.56458367
CTATCGA78900.014.37138217
AGGTGCG113750.014.12630466
TAGCAGA84250.014.1235529
CTCTAGC90950.013.8544926
CTAGTTG44300.013.8250378
TCTATCG83850.013.815145516
TCTAGCA143250.013.5102227
TGACTCT93550.013.4321153
TACTCTA45450.013.3999974
AGCGCGC46250.012.64042661
CACCTTA48550.012.6148137
CCTACAG79500.012.41410643
Produced by FastQC (version 0.11.5)