FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4697509
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG85750.18254355659563398No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT53870.1146778005108665No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC52910.11263416419212821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT17900.027.3817481
ACTCTAG25000.025.0614785
CTAGCAG21250.023.221448
GACTCTA23300.021.6319474
CTGACTC24200.019.8154282
CTCTAGC30650.017.3582846
GTGCGCA37100.016.89120568
TCTATCG28650.016.61263816
CTATCGA28850.016.49747317
GGTGCGC39000.016.24766267
TAGCAGA30000.015.8652399
TGCGCAT39600.015.82551569
TCTAGCA49950.015.4140287
GCGGGGG4550.015.38881
AGGTGCG41200.015.209866
TGACTCT32450.014.4537043
ATCTATC33650.014.04019215
GATCTAT34250.013.9985914
TAGCATG31900.013.9333627
TTCTAGC35400.013.54412324
Produced by FastQC (version 0.11.5)