FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_134-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_134-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7662146
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG141020.1840476545343824No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC88740.11581611731230389No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT87050.11361046892084802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT30750.026.1844861
ACTCTAG40000.023.2754425
CTAGCAG34900.022.8636768
GACTCTA39400.020.7871974
CTGACTC39500.020.2915252
GTGCGCA60300.017.7650168
TAGCAGA49050.016.8387419
GGTGCGC65250.016.57805867
TGCGCAT64500.016.5541669
CTATCGA46500.016.40752217
AGGTGCG67300.016.22870666
CTCTAGC51700.016.1125976
CCGGGGT6650.015.7928661
TCTATCG50850.015.1414816
TCTAGCA84600.014.8098037
TGACTCT57400.014.0245653
TAGCATG53600.013.9728727
AAGGTGC81650.013.71949565
CTAGCAT55350.013.65763826
GATCTAT56100.013.537168514
Produced by FastQC (version 0.11.5)