FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11938816
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG182960.15324802727506645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG61900.020.8655685
GCTGACT39950.020.331331
GTGCGCA78750.018.18101768
GGTGCGC82550.017.38628467
CTAGCAG48150.017.2265178
TGCGCAT83450.017.19906269
GACTCTA53750.016.8008124
AGGTGCG86950.016.62704366
CTGACTC55800.014.9919532
CTCTAGC71400.013.7753496
TCTAGCA110450.013.4986177
AAGGTGC111200.013.15848865
CTATCGA62550.013.1487417
GCGGGGC7750.013.1005911
GCGCATG117150.012.55030470
TACTCTA32950.012.5347484
TAGCAGA66000.012.5144469
CTAGTTG32450.012.29519758
TCTATCG66700.012.27811716
ATGCGCT118150.012.17538256
Produced by FastQC (version 0.11.5)