FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20324161
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG301630.1484095702646717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT70000.022.607221
ACTCTAG109250.021.65775
CTAGCAG86150.018.5242258
GACTCTA92000.018.3376714
TATGCCG33000.017.92273546
GTGCGCA129250.017.36192768
CTGACTC96950.016.8239252
GTATGCC35900.016.66990945
GGTGCGC136800.016.55677867
CGTATGC36650.016.42422744
TGCGCAT138050.016.2553569
ACGTCTG54850.015.75976515
GCACACG54950.015.60382211
AGGTGCG147900.015.31406766
CTATCGA106550.015.174623517
CCGGGGT15200.014.5112131
CTCTAGC121550.014.3980696
CGCGGGG13700.014.3111411
TCTATCG113800.014.17708616
GCGGGGG20750.014.0044741
Produced by FastQC (version 0.11.5)