FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13608606
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG220300.1618828555988762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT46150.022.0759411
ACTCTAG70350.019.4036525
CTAGCAG57800.017.9828688
GACTCTA65000.017.5544514
GTGCGCA95450.016.87146268
CGTATGC23000.016.28143544
GGTGCGC100350.016.2566667
CCGGGGT11200.016.254861
TATGCCG20950.016.20414446
CTGACTC65100.016.1299442
GTATGCC21500.015.95239645
TGCGCAT101900.015.90695969
AGGTGCG109950.014.90076766
GCACACG29850.014.77258711
CTCTAGC80500.014.4787066
CTATCGA70950.014.30471317
TCTATCG75700.013.77697616
TAGCAGA78150.013.3449719
TCTAGCA128200.013.2125827
CGCGGGG10000.012.9538731
Produced by FastQC (version 0.11.5)