FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12680033
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA333210.26278322777235674No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT189650.14956585680810136No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA184370.14540182979019062No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG166200.13107221408650907No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC149250.11770474098923875TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG40500.043.63873746
CGTATGC41050.042.45709644
GTATGCC43100.041.2498445
ATGCCGT48700.036.07563447
GCACACG57000.035.42705511
TCGTATG47750.034.81403443
ACGTCTG59600.034.29221715
ACACGTC61400.032.8881813
TGCCGTC52150.032.48119448
CACAGTG62850.032.1861233
ACACAGT63500.031.6913132
CACACAG65250.030.89474531
CTCGTAT41050.030.60637942
GTCACAC65900.030.4303329
CACGTCT67100.030.40733714
CACACGT66750.030.25219712
AGCACAC69850.029.26064910
ACTCTAG122600.029.2055575
CGTCTGA71350.028.59622216
CAGTCAC71050.028.37238927
Produced by FastQC (version 0.11.5)