FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12202651
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG247960.20320174689909595No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT142150.11649108050373644No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC136570.11191830365385358No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG126590.10373975294384803No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG124740.10222368893447827No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC122660.10051914129151117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT43550.024.5989571
ACTCTAG61600.021.4214925
GACTCTA57150.019.8433194
CTAGCAG57250.019.1951548
CTGACTC60150.017.6902562
GTGCGCA102550.017.13702668
GGTGCGC107600.016.26699667
TGCGCAT109150.016.22948869
AGGTGCG113500.015.45217766
TAGCAGA73200.015.0604159
CTATCGA72150.014.64894517
CTCTAGC77250.014.6350746
CCGGGGT9000.014.3927131
TCTATCG74750.014.18623516
TGACTCT83050.014.1611213
TCTAGCA130150.013.7408147
AGCGCGC41900.013.36733661
AAGGTGC137150.012.96634365
GCGCATG144100.012.87658370
GCCGGGT14250.012.7753231
Produced by FastQC (version 0.11.5)