FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10886180
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG216710.19906891122505782No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC112720.10354412658985981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT33300.022.2894781
ACTCTAG49700.018.099785
TATGCCG16000.017.71738846
CGTATGC15700.016.94129644
GACTCTA48150.016.9377674
GTGCGCA95000.016.80357268
CTAGCAG43900.016.741318
GGTGCGC98450.016.21449967
TGCGCAT98700.016.06731869
GTATGCC17200.015.667445
AGGTGCG104650.015.28717966
TCTAGCA108050.014.7373487
CTGACTC50300.014.6134172
CTCTAGC63300.013.2158386
AAGGTGC124000.013.12766965
CTCGTAT23800.012.646028542
ACTGACC22550.012.41629732
ATGCCGT23000.012.32508347
CTATCGA57950.012.32013417
ATGCGCT132200.012.28426356
Produced by FastQC (version 0.11.5)