FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13370961
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG195640.14631708222019346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23350.025.4805646
CGTATGC25850.023.42219744
GTATGCC26050.022.8395845
GCTGACT41250.021.812261
GCACACG33350.020.88322811
TAGGCAT31000.020.0959535
ACTTAGG33900.020.02844832
CACTTAG35100.019.64277331
ACTCTAG65050.019.6398415
ACGTCTG35900.019.10742215
ATGCCGT33300.018.392547
GACTCTA57200.017.6233674
ACACGTC39750.017.52096413
TCGTATG33900.017.44713643
CTAGCAG53800.017.3037328
CACACGT40300.017.0212512
TTAGGCA38250.017.01890834
GTGCGCA84850.016.95617568
CACGTCT42850.016.4984314
CTCGTAT33550.016.4816942
Produced by FastQC (version 0.11.5)