FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13263655
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG220800.16646995115599733No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT144310.10880108084837853No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC138840.10467702906928746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT46850.024.8827861
ACTCTAG74200.021.2740545
CTAGCAG61350.019.9660328
GACTCTA65800.019.04294
CTGACTC67850.017.693832
GTGCGCA99600.016.83587368
TGCGCAT105000.015.96996869
GGTGCGC105500.015.86091867
CTCTAGC83700.015.6396496
TAGCAGA79200.015.554499
AGGTGCG111500.014.97596466
CTATCGA79250.014.83782117
TCTATCG80700.014.65800716
TGACTCT94050.013.8067373
TCTAGCA138800.013.666167
TAGCATG87400.013.33437727
CTAGCAT89100.012.92284426
GCGGGCT12450.012.3722221
AAGGTGC139050.012.2856665
ATCTATC96350.012.24080415
Produced by FastQC (version 0.11.5)