FastQCFastQC Report
Thu 29 Nov 2018
HG7FMBGX9_n01_NCR_125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FMBGX9_n01_NCR_125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15039320
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG286820.19071340991480998No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT177660.11813034099946008No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC175620.11677389669213767No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG155180.10318285667171123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT53250.025.7721841
GTATGCC24600.022.47801645
TATGCCG25350.022.08917446
ACTCTAG80700.021.426235
CGTATGC26000.020.86377744
GACTCTA73150.020.1446694
CTAGCAG68850.019.8241838
CTGACTC73950.018.6013852
GTGCGCA121800.017.7618668
GGTGCGC126800.017.00631167
TGCGCAT127650.016.89329569
AGGTGCG132800.016.34344966
ATGCCGT34650.015.8574747
CTCTAGC93050.015.648436
CTATCGA87650.015.45216417
TAGCAGA92250.015.2888469
TCGTATG35500.015.28041143
CTCGTAT34500.015.21621942
TCTATCG91300.014.91107916
TGACTCT100850.014.7850623
Produced by FastQC (version 0.11.5)