FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454078
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30890.2124370219479285No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG2102.3519533E-919.7136275
GTATTAG5750.018.5993791
TAGGTAG3103.6379788E-1217.8058595
TCCTAAG3650.017.013132
TATACAG6900.016.9994345
TATTATA8000.016.8181882
GCTCGGA2702.923116E-916.6105586
CTCGGAG3209.822543E-1116.1713357
CCTAGAT2402.3107168E-715.8119723
CTAATAC5300.015.6221193
GTATAGA5750.015.599481
TAGACTG3101.0822987E-915.5801265
GTATTAT8500.015.0171461
CTAGGAC2302.5755326E-614.9994993
TACACAG4950.014.6358755
GTATATA9000.014.566181
TAATACT8800.014.5051984
TAGTCAG3353.2396201E-914.417439
CTATATA7450.014.3552254
CTATAGT3851.03682396E-1014.3371834
Produced by FastQC (version 0.11.2)