FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3955657
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117160.29618341529611897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14100.019.5748311
TAGGTAG8750.016.165815
GCTCGGA8450.015.1064056
GTATTAA16350.014.9819291
CGCGCAT5550.014.9186340
GTATAGA16550.014.8008781
GGCTCGG7400.014.4527885
TAATACT25550.014.4480444
CTAATAC16300.014.392543
TATACAG21550.014.2483435
TATTATA28400.013.7268462
CTCGGAG9300.013.7257137
TATAATA29350.013.5176262
ATTATAC20650.013.3655273
GTATTAT27650.013.351061
CATATAC20700.013.3332423
GTATAGG13400.013.130841
GTATAAT21300.013.1199621
GTCTAGT10850.013.0371071
TATACTG24350.013.0349855
Produced by FastQC (version 0.11.2)