FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892521
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33060.37041145250363855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG750.001990229623.00485854
GGCTCGG1653.1450327E-922.9971245
GCTCGGA1959.331416E-1021.2281156
TATTATA5750.020.3963052
TAGGTAG2201.9099389E-1020.3838165
CTCCTAA1701.0856638E-720.291581
TATTAGG3100.020.0286312
GTATTAG3300.019.8611531
CTCGGAG2102.3501343E-919.7118237
CCGAGCC1056.026616E-419.7118239
CTTACAC1952.1571395E-819.4580153
CAGGGCC1702.4339424E-618.2624239
TACACAG2701.5097612E-1017.8866525
CTAATAC3750.017.4768353
TAGTCAG1855.4506527E-616.7816859
GCCTATG1653.838622E-516.72799312
TATACCG2702.921297E-916.6090345
GTATAGA4000.016.385451
GTATACG4000.016.385451
TATACTG6550.016.3262025
Produced by FastQC (version 0.11.2)