FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5408541
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130400.24110014142446182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG10050.020.9398215
GTATTAG22650.020.4099431
TCCGCAC11250.018.40162364
GCTCGGA12200.018.0979486
TAGGTAG13400.018.0219765
TATTAGG22500.017.7856032
CTCGGAG12450.017.7345377
CGCACCT12800.016.44270566
TAATACT37850.016.1327554
GGGCTCG12250.016.0523994
CCGCACC13600.015.22164865
CTAATAC25100.014.8440083
TCTATAC24250.014.7952653
TATACTG33200.014.3400355
CCTAATA23400.014.1532
TTAGGTA19850.013.9037084
GTATATA43050.013.7033941
CGCGCAT6550.013.69371140
TATACAG31650.013.6252825
TAGTCAG13850.013.4513319
Produced by FastQC (version 0.11.2)