FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4192141
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79930.1906662967681669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA8700.019.0338926
TAGGTAG10250.018.511625
GGCTCGG8350.018.1790735
GTATTAG17150.017.9034461
TCCGCAC7750.017.80738464
CGCACCT9050.016.39312466
CCGCACC8700.016.25947465
CTCGGAG10300.015.74222857
TATACAG23750.014.6711235
TATATAC38150.014.1971243
CTAATAC18800.014.1295423
TAATACT29800.013.8918544
TATACCG11750.013.799575
GGGCTCG9500.013.7992424
TATTAGG18700.013.4671752
GTATATA35100.013.4655541
CCCCGGC5200.013.26834323
TAGTCAG10350.012.9997519
CCTAATA19950.012.9692112
TATAATA36050.012.8231522
Produced by FastQC (version 0.11.2)