FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n02_mini02_43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n02_mini02_43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225644
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27230.22216891691225182No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG2350.027.8925933
GCTCGGA2750.023.8354914
GTATTAG4950.022.3031231
CTCGGAG2950.022.2195245
GGGCTCG3200.020.4836242
TGGGCTC3650.017.9589791
ACCGTAT2652.2791937E-916.9239068
TACACCG2151.2758264E-616.0459235
CACCGCG2151.2763685E-616.04526723
TCGGAGA4100.015.9872196
TGGACCG1554.525682E-415.5800745
TATTAGG5450.015.1921032
CTTACAC2756.5761924E-815.0539943
GCCGTCT1851.0220747E-414.91776422
GGCACGC1709.124333E-414.20420236
TATACGG4152.1827873E-1114.1320125
GATGTGT5650.014.04487911
TAGACTG2951.5526166E-714.0333845
CTAATAC5700.013.9205433
TATACAG6700.013.9024765
Produced by FastQC (version 0.11.2)